Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein–Ligand Interactions. Issue 15 (16th March 2016)

• Record Type: Journal Article
• Authors: Guo, Yuan; Sakonsinsiri, Chadamas; Nehlmeier, Inga; Fascione, Martin A.; Zhang, Haiyan; Wang, Weili; Pöhlmann, Stefan; Turnbull, W. Bruce; Zhou, Dejian
• Is Part Of: Angewandte Chemie international edition. Volume 55:Issue 15(2016)
• Page Start: 4738
• Page End: 4742
• Publication Date: 2016-03-16
• Subjects: carbohydrates -- FRET -- proteins -- quantum dots -- viral inhibition; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201600593
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: A highly efficient cap‐exchange approach for preparing compact, dense polyvalent mannose‐capped quantum dots (QDs) has been developed. The resulting QDs have been successfully used to probe multivalent interactions of HIV/Ebola receptors DC‐SIGN and DC‐SIGNR (collectively termed as DC‐SIGN/R) using a sensitive, ratiometric Förster resonance energy transfer (FRET) assay. The QD probes specifically bind DC‐SIGN, but not its closely related receptor DC‐SIGNR, which is further confirmed by its specific blocking of DC‐SIGN engagement with the Ebola virus glycoprotein. Tuning the QD surface mannose valency reveals that DC‐SIGN binds more efficiently to densely packed mannosides. A FRET‐based thermodynamic study reveals that the binding is enthalpy‐driven. This work establishes QD FRET as a rapid, sensitive technique for probing structure and thermodynamics of multivalent protein–ligand interactions. Abstract : Glycoconjugates : Compact, dense polyvalent mannose‐capped quantum dots (QDs) have been prepared by a highly efficient ligand exchange approach. The quantum dots have been successfully employed to probe multivalent interactions of HIV/Ebola receptors (DC‐SIGN and DC‐SIGNR) using a rapid, sensitive, ratiometric readout strategy based on Förster resonance energy transfer.