Structure and Function of a "Head‐to‐Middle" Prenyltransferase: Lavandulyl Diphosphate Synthase. Issue 15 (29th February 2016)

• Record Type: Journal Article
• Authors: Liu, Meixia; Chen, Chun‐Chi; Chen, Lu; Xiao, Xiansha; Zheng, Yingying; Huang, Jian‐Wen; Liu, Weidong; Ko, Tzu‐Ping; Cheng, Ya‐Shan; Feng, Xinxin; Oldfield, Eric; Guo, Rey‐Ting; Ma, Yanhe
• Is Part Of: Angewandte Chemie international edition. Volume 55:Issue 15(2016)
• Page Start: 4721
• Page End: 4724
• Publication Date: 2016-02-29
• Subjects: biosynthesis -- fragrance -- monoterpenes -- natural products -- structure elucidation; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201600656
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: We report the first X‐ray structure of the unique "head‐to‐middle" monoterpene synthase, lavandulyl diphosphate synthase (LPPS). LPPS catalyzes the condensation of two molecules of dimethylallyl diphosphate (DMAPP) to form lavandulyl diphosphate, a precursor to the fragrance lavandulol. The structure is similar to that of the bacterial cis‐prenyl synthase, undecaprenyl diphosphate synthase (UPPS), and contains an allylic site (S1) in which DMAPP ionizes and a second site (S2) which houses the DMAPP nucleophile. Both S‐thiolo‐dimethylallyl diphosphate and S‐thiolo‐isopentenyl diphosphate bind intact to S2, but are cleaved to (thio)diphosphate, in S1. His78 (Asn in UPPS) is essential for catalysis and is proposed to facilitate diphosphate release in S1, while the P1 phosphate in S2 abstracts a proton from the lavandulyl carbocation to form the LPP product. The results are of interest since they provide the first structure and structure‐based mechanism of this unusual prenyl synthase. Abstract : Fragrance of lavender : The structure of lavandulyl diphosphate synthase, an irregular monoterpene synthase, was determined and is remarkably similar to the cis ‐prenyl synthases involved in bacterial cell wall biosynthesis. Determination of two ligand‐bound structures led to an unusual structure‐based mechanism of action.