Enhanced binding capacity of boronate affinity fibrous material for effective enrichment of nucleosides in urine samples. Issue 34 (18th March 2016)

• Record Type: Journal Article
• Authors: Gao, Li; Wang, Chaozhan; Wei, Yinmao
• Is Part Of: RSC advances. Volume 6:Issue 34(2016)
• Page Start: 28470
• Page End: 28476
• Publication Date: 2016-03-18
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra27898h
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A novel polyethyleneimine-modified boronate affinity fibrous cotton with high binding capacity was prepared for in-pipette-tip solid phase extraction nucleosides in human urine. Abstract : In this work, polyethyleneimine-modified boronate affinity fibrous cotton with good selectivity and higher binding capacity for cis -diols was developed for in-pipette-tip solid-phase extraction (SPE). The introduction of polyethyleneimine for the modification of cotton fiber provided abundant binding sites for adsorption of cis -diols, resulting in higher binding capacities up to 300 μg g −1 for catechol, 450 μg g −1 for dopamine and 700 μg g −1 for adenosine, respectively. The adsorbent could still show recognition ability towards cis -diols even with a 1000-fold amount of interferences in the solution. Several extraction parameters were optimized, including adsorbent dose, pipette times, washing and elution solvents. Under optimal extraction conditions, the proposed in-pipette-tip SPE method coupled with reversed-phase liquid chromatography was successfully used to extract nucleosides in urine. The limit of detection was 3.5–4.7 ng mL −1 and the recoveries were in the range of 89–102% with relative standard deviations of less than 9.9%. In conclusion, combination of the specific adsorption performance of the prepared boronate affinity fibrous material and in-pipette-tip SPE provided a fast pretreatment method of trace cis -diols in complex real samples.