Determining the isotopic abundance of a labeled compound by mass spectrometry and how correcting for natural abundance distribution using analogous data from the unlabeled compound leads to a systematic error. (24th February 2016)

• Record Type: Journal Article
• Authors: Schenk, David J.; Lockley, William J. S.; Elmore, Charles S.; Hesk, Dave; Roberts, Drew
• Is Part Of: Journal of labelled compounds & radiopharmaceuticals. Volume 59:Number 4(2016)
• Page Start: 136
• Page End: 146
• Publication Date: 2016-02-24
• Subjects: measurement error -- specific activity -- LC/MS -- mass spectrometry -- isotopic distribution -- natural abundance -- carbon‐13 -- carbon‐14 -- nitrogen‐15 -- IsoPat2 -- IsoCor -- Pynac -- natural abundance skew; Tracers (Chemistry) -- Periodicals; Radiopharmaceuticals -- Periodicals; 615.8424
• DOI: 10.1002/jlcr.3370
• Languages: English
• ISSNs: 0362-4803

Abstract

Abstract : When the isotopic abundance or specific activity of a labeled compound is determined by mass spectrometry (MS), it is necessary to correct the raw MS data to eliminate ion intensity contributions, which arise from the presence of heavy isotopes at natural abundance (e.g., a typical carbon compound contains ~1.1% 13 C per carbon atom). The most common approach is to employ a correction in which the mass‐to‐charge distribution of the corresponding unlabeled compound is used to subtract the natural abundance contributions from the raw mass‐to‐charge distribution pattern of the labeled compound. Following this correction, the residual intensities should be due to the presence of the newly introduced labeled atoms only. However, this will only be the case when the natural abundance mass isotopomer distribution of the unlabeled compound is the same as that of the labeled species. Although this may be a good approximation, it cannot be accurate in all cases. The implications of this approximation for the determination of isotopic abundance and specific activity have been examined in practice. Isotopically mixed stable‐atom labeled valine batches were produced, and both these and [ 14 C6 ]carbamazepine were analyzed by MS to determine the extent of the error introduced by the approach. Our studies revealed that significant errors are possible for small highly‐labeled compounds, such as valine, under some circumstances. In the case with [ 14 C6 ]carbamazepine, the errors introduced were minor but could be significant for 14 C‐labeled compounds with particular isotopic distributions. This source of systematic error can be minimized, although not eliminated, by the selection of an appropriate isotopic correction pattern or by the use of a program that varies the natural abundance distribution throughout the correction. Abstract : When determining the isotopic distribution or specific activity of a labeled compound by mass spectrometry (MS), systematic error may be introduced by the selection of an inappropriate natural abundance isotopic distribution for the correction of the raw MS mass‐to‐charge distribution. When the compound is highly labeled with carbon‐13 or carbon‐14, the error produced in the calculated isotopic distribution or specific activity of the labeled compound may be significant. In the practical cases tested, mixed isotopically labeled valine and nominally labeled [ 14 C6 ]carbamazepine, up to 40% error was observed in the relative abundance of particular isotopomers or in the tracer's specific activity depending on the correction pattern selected, the relative distribution of the isotopomers and/or the specific activity. The error can be minimized, although not eliminated, by the selection of an appropriate correction pattern and/or by the selection of an appropriate calculation program for the case in hand.