Dynamics of the T cell response to Mycobacterium tuberculosis DosR and Rpf antigens in a Colombian population of household contacts of recently diagnosed pulmonary tuberculosis patients. (March 2016)

• Record Type: Journal Article
• Authors: Arroyo, Leonar; Rojas, Mauricio; Ortíz, Blanca L.; Franken, Kees L.M.C.; García, Luis F.; Ottenhoff, Tom H.M.; Barrera, Luis F.
• Is Part Of: Tuberculosis. Volume 97(2016)
• Page Start: 97
• Page End: 107
• Publication Date: 2016-03
• Subjects: M. tuberculosis -- DosR -- Rpf -- RD-1 -- Latent tuberculosis infection -- T-cell response; 616.995
• DOI: 10.1016/j.tube.2015.12.008
• Languages: English
• ISSNs: 1472-9792

Abstract

Summary: Immune response to DosR and Rpf antigens from Mycobacterium tuberculosis ( Mtb ) seems to be important for latency maintenance. Little is known about the dynamics of the immune response to these antigens in an endemic community. Thus, the IFNγ response and cytokine production in response to PPD, Esat6-Cfp10 (E6-C10), DosR and Rpf antigens in healthy HHC of tuberculosis (TB) patients over a 12 (T12 ) months period (short-term, stLTBI) was investigated. This response was compared with a group of LTBI, who have remained healthy for 5–7 years (long-term, ltLTBI). According to the IFNγ response, two groups of HHCs were identified in stLTBI in response to E6-C10. At T12, E6-C10 + HHCs displayed a decrease in the IFNγ levels and a generalized decrease in cytokines production. The E6-C10 − HHC showed an increase in the IFNγ response and cytokine levels. In stLTBI, the responses to E6-C10, DosR, and Rpf may be interpreted as a protective immune response controlling Mtb infection and may be leading to a state of latent infection. Comparing the response of stLTBI and ltLTBI, we observed significant changes in the proportions of CD45RO + CD27 + T cells to specific DosR and Rpf, which may indicate a persistent immune response to Mtb antigens in ltLTBI.