The effects of an intraperitoneal single low dose of ketamine in attenuating the postoperative skin/muscle incision and retraction-induced pain related to the inhibition of N-methyl-d-aspartate receptors in the spinal cord. (11th March 2016)

• Record Type: Journal Article
• Authors: Shen, Yu; Xu, Li; Liu, Ming; Lei, Yishan; Gu, Xiaoping; Ma, Zhengliang
• Is Part Of: Neuroscience letters. Volume 616(2016)
• Page Start: 211
• Page End: 217
• Publication Date: 2016-03-11
• Subjects: CPOP chronic postoperative pain -- NMDA N-methyl-d-aspartate -- SMIR skin/muscle incision and retraction -- PWMT paw withdrawal threshold -- PWTL paw withdrawal latency -- CaMKII calcium/calmodulin-dependent kinase II -- PKA protein kinase A -- PKC protein kinase C -- LTP long-term potentiation; Chronic postoperative pain -- Central sensitization -- Skin/muscle incision and retraction -- N-methyl-d-aspartate; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2015.12.045
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: We showed the changing process of NMDARs at the spinal level of chronic postoperative pain. A single low dose ketamine could have a long-lasting effect in relieving chronic postoperative pain. This effect of ketamine might be associated with inhibiting the spinal NMDARs indirectly. Abstract: Background: Chronic postoperative pain (CPOP) is a common clinical problem which might be related to central sensitization. It has been widely accepted that NMDA ( N -methyl-d -aspartate) receptors are among the triggers of central sensitization. Ketamine is a non-competitive NMDA receptor antagonist that is widely used in alleviating postoperative pain, but its effect on CPOP has been rarely reported. In the present study, the skin/muscle incision and retraction (SMIR) model was used to investigate the role of NMDARs in chronic postoperative pain and the effect of an intraperitoneal single low dose ketamine (10 mg/kg) of attenuating SMIR-induced CPOP. Methods: We assessed pain behaviours after a SMIR operation by paw withdrawal threshold (PWMT) and paw withdrawal latency (PWMTL). Western blotting were performed to examine the role of NMDARs in SMIR-induced CPOP and the effect of ketamine on the expression and phosphorylation of NMDARs. Results: The SMIR operation induced long-lasting mechanical hyperalgesia, and the up-regulation of phosphorylated NMDARs and total NMDARs at the spinal level. A single intraperitoneal administration of low dose ketamine (10 mg/kg) during surgery alleviated pain behaviors and inhibited the up-regulation of phosphorylated NMDARs and total NMDARs. Conclusions: Our datas suggested that NMDARs play important roles in SMIR-induced CPOP. A single intraperitoneal low dose of ketamine could attenuate SMIR-induced CPOP, which might be associated with the inhibition of NMDARs. Our finding might provide a new, simple method of addressing CPOP.