Treatment study of distal femur for parathyroid hormone (1–34) and β-tricalcium phosphate on bone formation in critical-sized defects in osteopenic rats. Issue 10 (December 2015)

• Record Type: Journal Article
• Authors: Tao, Zhou-Shan; Zhou, Wan-Shu; Tu, Kai-kai; Huang, Zheng-Liang; Zhou, Qiang; Sun, Tao; Lv, Yang-Xun; Cui, Wei; Yang, Lei
• Is Part Of: Journal of cranio-maxillofacial surgery. Volume 43:Issue 10(2016:Mar.)
• Page Start: 2136
• Page End: 2143
• Publication Date: 2015-12
• Subjects: Defects -- β-TCP -- Micro-CT -- Osteoporosis -- PTH; Skull -- Surgery -- Periodicals; Maxilla -- Surgery -- Periodicals; Face -- Surgery -- Periodicals; Surgery, Plastic -- Periodicals; Maxilla -- surgery -- Periodicals; Face -- surgery -- Periodicals; Skull -- surgery -- Periodicals; Oral Surgical Procedures -- Periodicals; Reconstructive Surgical Procedures -- Periodicals; Surgery, Plastic -- Periodicals; Surgery, Oral -- Periodicals; Electronic journals; 617.514
• DOI: 10.1016/j.jcms.2015.09.004
• Languages: English
• ISSNs: 1010-5182

Abstract

Abstract: The objective of this study was to evaluate the effect of following combined treatment with parathyroid hormone (1–34) (PTH) and β-tricalcium phosphate (β-TCP) on local bone formation in a rat 3-mm critical-sized defect at the distal femur. Fourteen weeks were allowed to pass before defect surgery for the establishment of osteopenic animal models chronically fed a low-protein diet. All animals were randomly divided into four groups: group PTH; group β-TCP, group PTH + β-TCP, and a control group. All rats then underwent a surgical procedure to create bone defects in the bilateral distal femurs, and β-TCP was implanted into critical-sized defects for the groups designated as β-TCP and group PTH + β-TCP. After the defect operation, all animals from group PTH and group PTH + β-TCP received following subcutaneous injections with PTH (60 μg/kg, three times per week) until euthanasia at 4 and 8 weeks. The distal femurs and blood were collected for evaluation. The results of study showed the strongest effect on accelerating the local bone formation with treatment β-TCP and PTH at 4 weeks and 8 weeks. The results from our study demonstrate that a combination of PTH and β-TCP had an additive effect on local bone formation in osteopenic rats chronically fed a low-protein diet.