Α‐Galactosidase‐A Loaded‐Nanoliposomes with Enhanced Enzymatic Activity and Intracellular Penetration. Issue 7 (18th February 2016)

• Record Type: Journal Article
• Authors: Cabrera, Ingrid; Abasolo, Ibane; Corchero, José L.; Elizondo, Elisa; Gil, Pilar Rivera; Moreno, Evelyn; Faraudo, Jordi; Sala, Santi; Bueno, Dolores; González‐Mira, Elisabet; Rivas, Merche; Melgarejo, Marta; Pulido, Daniel; Albericio, Fernando; Royo, Miriam; Villaverde, Antonio; García‐Parajo, Maria F.; Schwartz, Simó; Ventosa, Nora; Veciana, Jaume
• Is Part Of: Advanced healthcare materials. Volume 5:Issue 7(2016)
• Page Start: 829
• Page End: 840
• Publication Date: 2016-02-18
• Subjects: α‐galactosidase A -- compressed CO2 -- Fabry disease -- nanoliposomes -- RGD targeting; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201500746
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract : Lysosomal storage disorders (LSD) are caused by lysosomal dysfunction usually as a consequence of deficiency of a single enzyme required for the metabolism of macromolecules, such as lipids, glycoproteins, and mucopolysaccharides. For instance, the lack of α‐galactosidase A (GLA) activity in Fabry disease patients causes the accumulation of glycosphingolipids in the vasculature leading to multiple organ pathology. Enzyme replacement therapy, which is the most common treatment of LSD, exhibits several drawbacks mainly related to the instability and low efficacy of the exogenously administered therapeutic enzyme. In this work, the unprecedented increased enzymatic activity and intracellular penetration achieved by the association of a human recombinant GLA to nanoliposomes functionalized with Arginine‐Glycine‐Aspartic acid (RGD) peptides is reported. Moreover, these new GLA loaded nanoliposomes lead to a higher efficacy in the reduction of the GLA substrate named globotriasylceramide in a cellular model of Fabry disease, than that achieved by the same concentration of the free enzyme. The preparation of these new liposomal formulations by DELOS‐SUSP, based on the depressurization of a CO2 ‐expanded liquid organic solution, shows the great potential of this CO2 ‐based methodology for the one‐step production of protein‐nanoliposome conjugates as bioactive nanomaterials with therapeutic interest. Abstract : α‐galactosidase‐A‐(GLA)‐loaded nano­liposomes functionalized with Arginine‐Glycine‐Aspartic acid (RGD) peptides are successfully prepared by using compressed CO2 . This nanoformulation shows an unprecedented increase of the GLA enzymatic activity and intracellular penetration, in comparison to the free enzyme. Moreover, these nanoconjugates lead to a higher efficacy in the reduction of the GLA substrate named globotriasylceramide (Gb3), in a cellular model of Fabry disease, than that achieved by the free enzyme.