In vitro dose and duration dependent approaches for the assessment of ameliorative effects of nanoconjugated vancomycin against Staphylococcus aureus infection induced oxidative stress in murine peritoneal macrophages. (February 2016)

• Record Type: Journal Article
• Authors: Chakraborty, Subhankari Prasad; Pramanik, Panchanan; Roy, Somenath
• Is Part Of: Microbial pathogenesis. Volume 91(2016)
• Page Start: 74
• Page End: 84
• Publication Date: 2016-02
• Subjects: Drug resistant Staphylococcus aureus -- Murine peritoneal macrophage -- Oxidative stress -- Antioxidant enzymes -- Nanoconjugated vancomycin; CAT Catalase -- CFU colony formation unit -- DNPH 2, 4-dinitrophenyl hydrazine -- DTNB 5′, 5′-dithio (bis)-2-nitrobenzoic acid -- EDTA ethylene diamine tetra acetate -- FBS fetal bovine serum -- GPx Glutathione peroxidase -- GR Glutathione reductase -- GSH reduced glutathione -- GSSG oxidized glutathione -- GST Glutathione-s-transferase -- MDA malondialdehyde -- MIC minimum inhibitory concentration -- MRSA methicillin resistant Staphylococcus aureus -- nuc nuclease -- NV nanoconjugated vancomycin -- PBS phosphate buffer saline -- PMA phorbol myristate acetate -- PMN polymorphonuclear neutrophils -- PRSA penicillin resistant Staphylococcus aureus -- S. aureus Staphylococcus aureus -- SD standard error of mean -- SOD Superoxide dismutase -- SSA sulfosalicylic acid -- TBA thiobutiric acid -- TBARS thiobutiric acid reactive substance -- TCA trichloro acetic acid -- VRSA vancomycin resistant Staphylococcus aureus -- VSSA vancomycin sensitive Staphylococcus aureus; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2015.11.001
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Aims: The present study was aimed to evaluate the in vitro ameliorative effect of nanoconjugated vancomycin (NV) against vancomycin sensitive and resistant strains of Staphylococcus aureus infection-induced oxidative stress in murine peritoneal macrophage. Methods: Peritoneal macrophages from mice were treated with VSSA and VRSA (5 × 10 6 CFU/mL), VSSA + NV (5–250 μg/ml) and VRSA + NV (5–250 μg/ml) for 18 h, having 3 h interval in culture media; and the superoxide anion generation, lipid peroxidation, protein oxidation, antioxidant enzymes status and glutathione enzymes activity were monitored. Results: The significantly increased free radical generation, lipid peroxidation, protein carbonyls and oxidized glutathione levels were observed in VSSA and VRSA treated group as compared to control group; where as reduced glutathione level, antioxidant enzymes status and glutathione dependent enzymes were decreased significantly. All these changes come near to control in NV treated group in a dose and duration dependent fashion. Among the different doses and duration intervals of NV, maximum ameliorative effect was observed by 100 μg/ml for 12 h treatment which does not produce any damage to the cell. Conclusions: These findings suggest the potential use and beneficial role of nanoconjugated vancomycin as a modulator of S. aureus infection-induced cellular damage in murine peritoneal macrophage. Graphical abstract: Highlights: Infection of vancomycin sensitive and resistant strain of Staphylococcus aureus in murine peritoneal macrophage. S. aureus infection-induced oxidative stress in murine peritoneal macrophages. In vitro ameliorative role of nanoconjugated vancomycin (NV) against S. aureus infection-induced oxidative stress in murine peritoneal macrophages.