A highly specific and sensitive loop-mediated isothermal amplification method for the detection of Escherichia coli O157:H7. (February 2016)

• Record Type: Journal Article
• Authors: Ravan, Hadi; Amandadi, Mojdeh; Sanadgol, Nima
• Is Part Of: Microbial pathogenesis. Volume 91(2016)
• Page Start: 161
• Page End: 165
• Publication Date: 2016-02
• Subjects: Loop-mediated isothermal amplification -- LAMP assay -- Escherichia coli O157:H7 -- Z3276 gene; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2015.12.011
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: E. coli O157:H7 is one of the most important foodborne pathogen that causes some human illnesses such as bloody diarrhea, hemolytic-uremic syndrome, and kidney failure. We developed a loop-mediated isothermal amplification (LAMP) assay with six special primers that target a highly specific 299-bp region of the Z3276 gene for the detection of E. coli O157:H7. Among 117 bacterial strains tested in this study, positive results were only obtained from E. coli O157:H7 strains. The sensitivity level of the Z3276-LAMP assay was determined to be 5 CFU/reaction tube in pure bacterial culture. Moreover, the LAMP assay was successfully applied to artificially contaminated ground beef with a sensitivity level of 10 3 CFU/mL without pre-enrichment and 10 CFU/mL after a 4-h pre-enrichment. In conclusion, the present LAMP assay would be a useful and powerful tool for the rapid, sensitive, and specific diagnosis of E. coli O157:H7 strains in resource limited laboratories. Highlights: Detecting of Escherichia coli O157:H7. A highly sensitive and specific LAMP assay for detecting E. coli O157:H7. The LAMP assay can be used for the detection of E. coli O157:H7 in food staffs.