Dextran sulfate sodium upregulates MAPK signaling for the uptake and subsequent intracellular survival of Brucella abortus in murine macrophages. (February 2016)

• Record Type: Journal Article
• Authors: Reyes, Alisha Wehdnesday Bernardo; Arayan, Lauren Togonon; Simborio, Hannah Leah Tadeja; Hop, Huynh Tan; Min, WonGi; Lee, Hu Jang; Kim, Dong Hee; Chang, Hong Hee; Kim, Suk
• Is Part Of: Microbial pathogenesis. Volume 91(2016)
• Page Start: 68
• Page End: 73
• Publication Date: 2016-02
• Subjects: Brucella abortus -- Dextran sulfate sodium -- Adhesion -- Internalization -- Macrophage; DSS dextran sulfate sodium -- MAPKs mitogen-activated protein kinases -- BCVs Brucella-containing vacuoles; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2015.10.024
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Brucellosis is one of the major zoonoses worldwide that inflicts important health problems in animal and human. Here, we demonstrated that dextran sulfate sodium (DSS) significantly increased adhesion of Brucella ( B. ) abortus in murine macrophages compared to untreated cells. Even without infection, Brucella uptake into macrophages increased and F-actin reorganization was induced compared with untreated cells. Furthermore, DSS increased the phosphorylation of MAPKs (ERK1/2 and p38α) in Brucella- infected, DSS-treated cells compared with the control cells. Lastly, DSS markedly increased the intracellular survival of Brucella abortus in macrophages by up to 48 h. These results suggest that DSS enhanced the adhesion and phagocytosis of B. abortus into murine macrophages by stimulating the MAPK signaling proteins phospho-ERK1/2 and p38α and that DSS increased the intracellular survival of B. abortus by inhibiting colocalization of Brucella -containing vacuoles (BCVs) with the late endosome marker LAMP-1. This study emphasizes the enhancement of the phagocytic and intracellular modulatory effects of DSS, which may suppress the innate immune system and contribute to prolonged Brucella survival and chronic infection. Highlights: DSS has no bactericidal effect against B. abortus. DSS increased adherence, uptake and intracellular growth of B. abortus in macrophages. DSS activated F-actin polymerization and MAPKs signaling in macrophages. DSS decreased colocalization of BCVs with LAMP-1. DSS may contribute to prolonged survival and chronic Brucella infection.