Protective effect of Naoxintong against cerebral ischemia reperfusion injury in mice. (22nd April 2016)

• Record Type: Journal Article
• Authors: Xue, Jing; Zhang, Xiangjian; Zhang, Cong; Kang, Ning; Liu, Xiaoxia; Yu, Jingying; Zhang, Nan; Wang, Hong; Zhang, Lan; Chen, Rong; Cui, Lili; Wang, Lina; Wang, Xiaolu
• Is Part Of: Journal of ethnopharmacology. Volume 182(2016)
• Page Start: 181
• Page End: 189
• Publication Date: 2016-04-22
• Subjects: NXT Naoxintong -- tMCAO Transient Middle Cerebral Artery Occlusion -- IHC Immunohistochemistry -- qRT-PCR Quantitative Reverse Transcription-Polymerase Chain Reaction -- LOX-1 Lectin-like Oxidized Low-Density Lipoprotein Receptor-1 -- ERK Extracellular Regulated Protein Kinases -- ROS Reactive Oxygen Species -- LDL Low Density Lipoproteins -- NF-κB Nuclear Factor-Kappa B -- I/R Ischemia Reperfusion -- MAPKs Mitogen-Activated Kinases -- TTC Triphenyl Tetrazolium Chloride -- MDA Malondialdehyde -- SOD Superoxide Dismutase -- BYHWD Bu-Yang-Huan-Wu Decoction; Paeoniflorin (PubChem CID: 442534) -- Tanshinone IIA (PubChem CID: 164676) -- Salvianolic acid B (PubChem CID: 6451084); Cerebral ischemia reperfusion -- Neuroprotection -- Naoxintong -- LOX-1 -- ERK1/2 -- NF-κB; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2016.02.022
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Naoxintong (NXT), a renowned traditional Chinese medicine in China, has been used for the treatment of acute and chronic cardio-cerebrovascular diseases in clinic for more than 20 years. Aim of the study: To evaluate the potential neuroprotective effect of NXT against ischemia reperfusion (I/R) injury in mice and investigate the underlying mechanisms. Materials and methods: Focal cerebral I/R injury in adult male CD-1 mice was induced by transient middle cerebral artery occlusion (tMCAO) for 1 h followed by reperfusion for 23 h. Mice were randomly divided into five groups: Sham group; tMCAO group; Vehicle group; NXT-treated groups at doses of 0.36 g/kg and 0.54 g/kg. The effects of NXT on murine neurological function were estimated by neurological defect scores, infarct volume and brain water content at 24 h after tMCAO. Immunohistochemistry and Western blot were used to detect the expression of LOX-1, pERK1/2 and NF-κB at 24 h after tMCAO. qRT-PCR was used to detect the expression of LOX-1 and NF-κB at 24 h after tMCAO. Results: Compared with Vehicle group, 0.54 g/kg group of NXT significantly ameliorated neurological outcome, infarction volume and brain water content, decreased the expression of LOX-1, pERK1/2 and NF-κB ( P <0.05). Conclusion: NXT protected the mice brain against I/R injury, and this protection maybe associated with the down-regulation of LOX-1, pERK1/2 and NF-κB expression. Graphical abstract: