Photo-induced coupling reactions of tetrazoles with carboxylic acids in aqueous solution: application in protein labelling. Issue 25 (8th March 2016)

• Record Type: Journal Article
• Authors: Zhao, Shan; Dai, Jianye; Hu, Mo; Liu, Chang; Meng, Rong; Liu, Xiaoyun; Wang, Chu; Luo, Tuoping
• Is Part Of: Chemical communications. Volume 52:Issue 25(2016)
• Page Start: 4702
• Page End: 4705
• Publication Date: 2016-03-08
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc10445a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : The coupling reactions of diaryltetrazoles with carboxylic acids under UV irradiation were investigated. Application of these transformations in chemical biology was demonstrated in photo-labelling the proteinogenic carboxylic acids in purified proteins, cell lysates and living cells. Abstract : The photo-induced reactions of diaryltetrazoles with carboxylic acids in aqueous solution were investigated. Besides measuring the apparent second-order rate constant and evaluating the functional group compatibility of these reactions, we further incorporated the tetrazoles into SAHA, leading to a new active-site-directed probe for labelling HDACs in both cell lysates and living cells.