Comparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity properties. (April 2016)

• Record Type: Journal Article
• Authors: Silva, Maria Elisabete F.; Lopes, Ana R.; Cunha-Queda, Ana Cristina; Nunes, Olga C.
• Is Part Of: Waste management. Volume 50(2016)
• Page Start: 20
• Page End: 30
• Publication Date: 2016-04
• Subjects: Bacterial community -- Diversity -- DGGE profiling -- 454-pyrosequencing -- Multivariate analysis; Hazardous wastes -- Periodicals; Refuse and refuse disposal -- Periodicals; 363.728
• DOI: 10.1016/j.wasman.2016.02.023
• Languages: English
• ISSNs: 0956-053X

Abstract

Highlights: Composts with different physicochemical properties also had different bacterial communities. EC, Cu, OM contents, GI, and fungi counts were related with the bacterial communities. The mature compost had a more diverse bacterial community than the immature. Pseudonocardiaceae, Streptomycetaceae and Bacillaceae were abundant in the mature compost. Aerococcaceae and Corynebacteriaceae prevailed in the immature compost. Abstract: Previously, two municipal solid waste commercial composts (MSW1 and MSW2) were characterized. Although sharing the same type of raw material, most of their physicochemical, stability and maturity properties differed. The present study aimed to characterize them at a microbiological level, and to infer on possible relationships between the composts properties and the structure of their bacterial communities. Both the 16S rRNA gene-based PCR-DGGE profiling and 454-pyrosequencing technology showed that the structure of the bacterial communities of these composts was distinct. The bacterial community of MSW1 was more diverse than that of MSW2. Multivariate analyses revealed that the high electrical conductivity, Cu content as well as the low phytotoxity of compost MSW1, when compared to MSW2, contributed most to shape its bacterial community structure. Indeed, high abundance of halophilic ( Halomonadaceae and Brevibacteriaceae ) and metal resistant organisms ( Brevibacteriaceae and Bacillaceae ) were found in MSW1. In addition, Pseudonocardiaceae, Streptomycetaceae, Bacillaceae, and Brevibacteriaceae may have contributed to the high humic-like acids content and low phytotoxicity of MSW1. In contrast, the high organic matter content and the high density of the cultivable fungi population were the parameters most correlated with the structure of the bacterial community of compost MSW2, dominated by Corynebacteriaceae and mainly Aerococcaceae, taxonomic groups not commonly found in composts.