Characterization of senescence-associated protease activities involved in the efficient protein remobilization during leaf senescence of winter oilseed rape. (May 2016)

• Record Type: Journal Article
• Authors: Poret, Marine; Chandrasekar, Balakumaran; van der Hoorn, Renier A.L.; Avice, Jean-Christophe
• Is Part Of: Plant science. Volume 246(2016:May)
• Page Start: 139
• Page End: 153
• Publication Date: 2016-05
• Subjects: AP aspartic protease -- CCV chloroplast vesiculation-containing vesicle -- CP cysteine protease -- CXE carboxylesterase -- HN high nitrate -- LN low nitrate -- MES methylesterase -- N nitrogen -- NPC no probe control -- NRE nitrogen remobilization efficiency -- NUE nitrogen use efficiency -- PLCP papain-like cysteine protease -- POPL prolyloligopeptidase-like protease -- RuBisCO (RBC) ribulose-1, 5-biphosphate carboxylase/oxygenase -- RBCL large subunit of RuBisCO -- SAP senescence associated protease -- SAV senescence associated vesicles -- SCPL serine carboxypeptidase-like protein -- VPE vacuolar processing enzyme; Brassica napus -- Nitrogen remobilization efficiency -- Soluble proteins -- Protease activity -- Senescence-associated proteases -- Cysteine proteases; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2016.02.011
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Aspartic proteases and proteasome were active during leaf senescence in oilseed rape. Activities of serine proteases increased when protein remobilization occurred in leaf. High protein remobilization is linked to an increase of cysteine proteases activities. Papain-like Cys proteases and vacuolar processing enzymes are the major cys proteases Abstract: Oilseed rape ( Brassica napus L.) is a crop plant characterized by a poor nitrogen (N) use efficiency that is mainly due to low N remobilization efficiency during the sequential leaf senescence of the vegetative stage. As a high leaf N remobilization efficiency was strongly linked to a high remobilization of proteins during leaf senescence of rapeseed, our objective was to identify senescence-associated protease activities implicated in the protein degradation. To reach this goal, leaf senescence processes and protease activities were investigated in a mature leaf becoming senescent in plants subjected to ample or low nitrate supply. The characterization of protease activities was performed by using in vitro analysis of RuBisCO degradation with or without inhibitors of specific protease classes followed by a protease activity profiling using activity-dependent probes. As expected, the mature leaf became senescent regardless of the nitrate treatment, and nitrate limitation enhanced the senescence processes associated with an enhanced degradation of soluble proteins. The characterization of protease activities revealed that: (i) aspartic proteases and the proteasome were active during senescence regardless of nitrate supply, and (ii) the activities of serine proteases and particularly cysteine proteases (Papain-like Cys proteases and vacuolar processing enzymes) increased when protein remobilization associated with senescence was accelerated by nitrate limitation. Short statement: Serine and particularly cysteine proteases (both PLCPs and VPEs) seem to play a crucial role in the efficient protein remobilization when leaf senescence of oilseed rape was accelerated by nitrate limitation.