Extract from spent hop (Humulus lupulus L.) reduces blood platelet aggregation and improves anticoagulant activity of human endothelial cells in vitro. (April 2016)

• Record Type: Journal Article
• Authors: Luzak, Boguslawa; Golanski, Jacek; Przygodzki, Tomasz; Boncler, Magdalena; Sosnowska, Dorota; Oszmianski, Jan; Watala, Cezary; Rozalski, Marcin
• Is Part Of: Journal of functional foods. Volume 22(2016)
• Page Start: 257
• Page End: 269
• Publication Date: 2016-04
• Subjects: Spent hops -- Humulus lupulus L. -- Endothelium -- Blood platelet -- CD39 -- Cardiovascular disorders; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.01.029
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Spent hop extract exerts significant beneficial effects on haemostatic system. Spent hop extract has strong anti-platelet activity. Spent hop extract improves anticoagulant activity of human endothelial cells. Feeding of diabetic rats with spent hop extract significantly prolongs their lifespan. Spent hop extract can play a role in nutritional vascular protection. Abstract: Spent hop extract (SHE) is hypothesized to possess multifaceted anticoagulant effect and to reduce platelet reactivity in a direct manner and to improve the antiplatelet action of endothelium. The results demonstrated that SHE, rich in flavanols (50% of total phenolic compounds), hydroxycinnamic acids, proanthocyanidin oligomers, flavan-3-ol monomers, and flavonol glycosides, inhibited ADP-induced platelet aggregation up to 11% for 7.5 µg/ml or up to 23% for 15 µg/ml, P < 0.05. Additionally, SHE ameliorated anticoagulant activity of human endothelial cells to significantly reduce platelet reactivity. This effect could be related to the increased expression/activity of the ecto-ADPase (CD39). ADP-induced platelet reactivity was reduced in the case of platelets incubated with the extract-treated endothelial cells compared to the untreated cells. Diabetic rats fed with SHE demonstrated longer survival time than the group treated with placebo. In conclusion, our study provided evidence for a potential beneficial role of SHE in the regulation of platelet and endothelial function.