Down-regulation apoptosis signal-regulating kinase 1 gene reduced the Litopenaeus vannamei hemocyte apoptosis in WSSV infection. (March 2016)

• Record Type: Journal Article
• Authors: Yuan, Feng-Hua; Chen, Yong-Gui; Zhang, Ze-Zhi; Yue, Hai-Tao; Bi, Hai-Tao; Yuan, Kai; Weng, Shao-Ping; He, Jian-Guo; Chen, Yi-Hong
• Is Part Of: Fish & shellfish immunology. Volume 50(2016:May)
• Page Start: 109
• Page End: 116
• Publication Date: 2016-03
• Subjects: Apoptosis signal-regulating kinase 1 -- Litopenaeus vannamei -- White spot syndrome virus -- Apoptosis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2015.12.003
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Apoptosis signal-regulating kinase 1 (ASK1), a mitogen-activated protein kinase kinase kinase, is crucial in various cellular responses. In the present study, we identified and characterized an ASK1 homolog from Litopenaeus vannamei ( LvASK1 ). The full-length cDNA of LvASK1 was 5400 bp long, with an open reading frame encoding a putative 1420 amino acid protein. LvASK1 was highly expressed in muscle, hemocyte, eyestalk and heart. Real-time RT-PCR analysis showed that the expression of the LvASK1 was upregulated during the white spot syndrome virus (WSSV) challenge. The knocked-down expression of LvASK1 by RNA interference significantly reduced the apoptotic ratio of the hemocytes collected from WSSV-infected L . vannamei . Furthermore, the down-regulation of LvASK1 also decreased the cumulative mortality of WSSV-infected L . vannamei . These results suggested that down-regulation of LvASK1 decreased the apoptotic rate of hemocytes in WSSV-infected shrimp, and that it could contribute to the reduction of cumulative mortality in WSSV-infected L . vannamei . Highlights: Identification of apoptosis signal-regulating kinase 1 in Litopenaeus vannamei (designated as LvASK-1). Knocked-down LvASK-1 caused a lower shrimp hemocyte apoptotic ratio of L . vannamei upon WSSV infection. Knocked-down LvASK-1 result in a lower cumulative mortality of L . vannamei upon WSSV infection.