Proteomic analysis by iTRAQ in red claw crayfish, Cherax quadricarinatus, hematopoietic tissue cells post white spot syndrome virus infection. (March 2016)

• Record Type: Journal Article
• Authors: Jeswin, Joseph; Xie, Xiao-lu; Ji, Qiao-lin; Wang, Ke-jian; Liu, Hai-peng
• Is Part Of: Fish & shellfish immunology. Volume 50(2016:May)
• Page Start: 288
• Page End: 296
• Publication Date: 2016-03
• Subjects: Crayfish hematopoietic tissue cells -- White spot syndrome virus -- iTRAQ -- Proteomics; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.01.035
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: To elucidate proteomic changes of Hpt cells from red claw crayfish, Cherax quadricarinatus, we have carried out isobaric tags for relative and absolute quantitation (iTRAQ) of cellular proteins at both early (1 hpi) and late stage (12 hpi) post white spot syndrome virus (WSSV) infection. Protein database search revealed 594 protein hits by Mascot, in which 17 and 30 proteins were present as differentially expressed proteins at early and late viral infection, respectively. Generally, these differentially expressed proteins include: 1) the metabolic process related proteins in glycolysis and glucogenesis, DNA replication, nucleotide/amino acid/fatty acid metabolism and protein biosynthesis; 2) the signal transduction related proteins like small GTPases, G-protein-alpha stimulatory subunit, proteins bearing PDZ- or 14-3-3-domains that help holding together and organize signaling complexes, casein kinase I and proteins of the MAP-kinase signal transduction pathway; 3) the immune defense related proteins such as α-2 macroglobulin, transglutaminase and trans -activation response RNA-binding protein 1. Taken together, these protein information shed new light on the host cellular response against WSSV infection in a crustacean cell culture. Highlights: Among 594 proteins, 17 and 30 differentially expressed proteins at early and late viral infection, respectively. Differentially expressed proteins associated to metabolic process, signal transduction, immune defense and cytoskeleton. The study shed new light on the host cellular response against WSSV infection in a crustacean cell culture.