Introducing a protocol to create bisphosphonate-related osteonecrosis of the jaw in rat animal model. Issue 3 (March 2016)

• Record Type: Journal Article
• Authors: Zandi, Mohammad; Dehghan, Arash; Malekzadeh, Hamid; Janbaz, Pejman; Ghadermazi, Khaled; Amini, Payam
• Is Part Of: Journal of cranio-maxillofacial surgery. Volume 44:Issue 3(2016)
• Page Start: 271
• Page End: 278
• Publication Date: 2016-03
• Subjects: Bisphosphonate -- Osteonecrosis -- Jaw -- Zoledronate -- Animal study; Skull -- Surgery -- Periodicals; Maxilla -- Surgery -- Periodicals; Face -- Surgery -- Periodicals; Surgery, Plastic -- Periodicals; Maxilla -- surgery -- Periodicals; Face -- surgery -- Periodicals; Skull -- surgery -- Periodicals; Oral Surgical Procedures -- Periodicals; Reconstructive Surgical Procedures -- Periodicals; Surgery, Plastic -- Periodicals; Surgery, Oral -- Periodicals; Electronic journals; 617.514
• DOI: 10.1016/j.jcms.2015.12.010
• Languages: English
• ISSNs: 1010-5182

Abstract

Abstract: Objective: Previously published animal investigations on bisphosphonate-related osteonecrosis of the jaws (BRONJ) showed a variety of methods for BRONJ induction and inconsistent findings. The aim of present study was to develop a reliable protocol for BRONJ induction in rat animal model. Subjects and methods: In a pilot study, 64 rats were randomly divided into 4 groups and 16 subgroups (each containing 2 experimental and 2 control rats) based on the timing of tooth extraction and euthanasia. The experimental and control rats received intraperitoneal injection of 0.06 mg/kg zoledronate and saline, respectively, once a week until sacrificed, and evaluated for presence of bone exposure clinically, and osteonecrosis and new bone formation histologically. The protocol that successfully produced BRONJ in pilot study was tested in a randomized controlled experimental investigation using 45 rats. Results: In pilot investigation, the highest rate of BRONJ was obtained after four weekly zoledronate injections, at least 4 weeks after tooth extraction. The randomized controlled experimental study verified this finding with a success rate of 83%, and also showed that more prolongation of zoledronate therapy did not increase the BRONJ rate. Conclusion: The protocol developed in the present study could be used reliably for future BRONJ investigations on rats.