Layer‐by‐Layer Assembly of Metal–Organic Frameworks in Macroporous Polymer Monolith and Their Use for Enzyme Immobilization. Issue 6 (25th January 2016)

• Record Type: Journal Article
• Authors: Wen, Liyin; Gao, Aicong; Cao, Yao; Svec, Frantisek; Tan, Tianwei; Lv, Yongqin
• Is Part Of: Macromolecular rapid communications. Volume 37:Issue 6(2016)
• Page Start: 551
• Page End: 557
• Publication Date: 2016-01-25
• Subjects: enzyme immobilization -- layer growth -- macroporous polymer monolith -- metal–organic framework -- self‐assembly; Macromolecules -- Periodicals; Polymers -- Periodicals; Chemistry -- Periodicals; 547.705
• DOI: 10.1002/marc.201500705
• Languages: English
• ISSNs: 1022-1336

Abstract

Abstract : New monolithic materials comprising zeolitic imidazolate framework (ZIF‐8) located on the pore surface of poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate) monolith previously functionalized with N ‐(3‐aminopropyl)‐imidazole have been prepared via a layer‐by‐layer self‐assembly strategy. These new ZIF‐8@monolith hybrids are used as solid‐phase carriers for enzyme immobilization. Their performance is demonstrated with immobilization of a model proteolytic enzyme trypsin. The best of the conjugates enable very efficient digestion of proteins that can be achieved in mere 43 s. Abstract : A new metal–organic polymer monolith hybrid is developed via a layer‐by‐layer self‐assembly strategy, which renders excellent performances as solid carriers for enzyme immobilization as demonstrated from the high proteolytic efficiency for digestion of proteins in a very short time.