Congenital macrothrombocytopenia‐linked mutations in the actin‐binding domain of α‐actinin‐1 enhance F‐actin association. Issue 6 (1st March 2016)

• Record Type: Journal Article
• Authors: Murphy, Anita C. H.; Lindsay, Andrew J.; McCaffrey, Mary W.; Djinović‐Carugo, Kristina; Young, Paul W.
• Is Part Of: FEBS letters. Volume 590:Issue 6(2016)
• Page Start: 685
• Page End: 695
• Publication Date: 2016-03-01
• Subjects: actin -- actinin‐1 -- ACTN1 -- alpha‐actinin -- congenital macrothrombocytopenia -- macrothrombocytopenia -- α‐actinin; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.12101
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Mutations in the actin cross‐linking protein actinin‐1 were recently linked to dominantly inherited congenital macrothrombocytopenia. Here, we report that several disease‐associated mutations that are located within the actinin‐1 actin‐binding domain cause increased binding of actinin‐1 to actin filaments and enhance filament bundling in vitro . These actinin‐1 mutants are also more stably associated with the cytoskeleton in cultured cells, as assessed by biochemical fractionation and fluorescence recovery after photobleaching experiments. For two mutations the disruption of contacts between the calponin homology domains within the actinin actin‐binding domain may explain increased filament binding – providing mechanistic and structural insights into the basis of actinin‐1 dysfunction in congenital macrothrombocytopenia. Abstract :