Immunological evaluation of the entirely carbohydrate-based Thomsen-Friedenreich – PS B conjugate12. Issue 13 (9th March 2016)

• Record Type: Journal Article
• Authors: Trabbic, Kevin R.; Bourgault, Jean-Paul; Shi, Mengchao; Clark, Matthew; Andreana, Peter R.
• Is Part Of: Organic & biomolecular chemistry. Volume 14:Issue 13(2016)
• Page Start: 3350
• Page End: 3355
• Publication Date: 2016-03-09
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c6ob00176a
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : PS B, a CD 4+ T-cell stimulating zwitterionic polysaccharide from B. fragilis, was conjugated with aminooxy TF tumor antigen. Immunization revealed Ab specificity to TF. FACS revealed Ab binding to MCF-7 but not HCT-116 cells. Abstract : PS B, a naturally occurring CD4 + T-cell simulating zwitterionic polysaccharide from Bacteroides fragilis ATCC 25285/NCTC 9343, was conjugated with aminooxy Thomsen Friedenreich (TF or T) [α-d -Gal-(1, 3)-β-d -GalNAc-ONH2 ] tumor antigen. Immunization in Jax C57BL/6, followed by ELISA revealed IgM and IgG antibody TF specificity. FACS data noted preferential binding to TF-laced MCF-7 cells but not to HCT-116 cells.