Expression of G1/S‐cyclins and cyclin‐dependent kinase inhibitors in actinic keratosis and squamous cell carcinoma. (19th October 2015)

• Record Type: Journal Article
• Authors: Brasanac, Dimitrije; Stojkovic‐Filipovic, Jelena; Bosic, Martina; Tomanovic, Nada; Manojlovic‐Gacic, Emilija
• Is Part Of: Journal of cutaneous pathology. Volume 43:Number 3(2016)
• Page Start: 200
• Page End: 210
• Publication Date: 2015-10-19
• Subjects: cyclin D1 -- cyclin E -- p16 -- p21 -- squamous cell carcinoma; Skin -- Diseases -- Periodicals; Dermatology -- Periodicals; 616
• DOI: 10.1111/cup.12623
• Languages: English
• ISSNs: 0303-6987

Abstract

Abstract : Background: Actinic keratosis (AK) and Bowen's disease (squamous cell carcinoma in situ, SCCIS) are pre‐invasive stages in the development of squamous cell carcinoma (SCC). Methods: Immunohistochemical study of cyclin D1, cyclin E, p16 INK4a and p21 Cip1 /Waf1 in AK (53 cases), SCCIS (16 cases) and SCC (40 cases), in relation to the type of the lesion and SCC prognostic parameters (grade, diameter and thickness). Results: Diffuse cyclin D1 distribution was more frequent in SCCIS and SCC than in AK (p = 0.03) and similar pattern was observed for p16 INK4a . For cyclin E, central distribution dominated in SCC compared with the AK (p = 0.001) and SCCIS (p = 0.03). p21 Cip1 /Waf1 displayed suprabasal distribution more frequently in AK than in SCCIS (p = 0.001) and SCC (p = 0.0004). Semiquantitative assessment showed more positive cells in AK (p = 0.04) and SCCIS (p = 0.04) than in SCC for cyclin E. SCC with diameter over 20 mm and those thicker than 6 mm revealed higher labeling index with p16 INK4a and p21 Cip1 /Waf1, respectively. Conclusions: Our results suggest different alterations for p16 INK4a and p21 Cip1 /Waf1 in AK, SCCIS and SCC. Immunostaining distribution showed closer correlation with the type of the lesion, whereas percentage of positive cells displayed better association with the SCC prognostic parameters.