A higher frequency of IL‐10‐producing B cells in Hepatitis B virus‐associated membranous nephropathy. (April 2016)

• Record Type: Journal Article
• Authors: Liu, Yong; Wang, Haifeng; Hu, Xintong; Qu, Zhihui; Zhang, Huimao; Crew, Rebecca; Ayana, Desalegn Admassu; Li, Man; Qiu, Jinpeng; Jiang, Yanfang
• Is Part Of: Clinical and experimental pharmacology and physiology. Volume 43:Number 4(2016:Apr.)
• Page Start: 417
• Page End: 427
• Publication Date: 2016-04
• Subjects: breg cell -- HBV‐MN -- IL‐10 -- plasma cell; Clinical pharmacology -- Periodicals; Pharmacology, Experimental -- Periodicals; Physiology, Experimental -- Periodicals; Physiology, Pathological -- Periodicals; 615.1
• DOI: 10.1111/1440-1681.12552
• Languages: English
• ISSNs: 0305-1870

Abstract

Summary: The purpose of this study is to elucidate the potential role of interleukin (IL)‐10 + regulatory B cells and other B cell subsets in the development of hepatitis B virus‐associated membranous nephropathy (HBV‐MN). A total of 14 patients with new onset HBV‐MN, 12 individuals with immune‐tolerant HBV infection (HBV‐IT), and 12 healthy controls (HC) were examined for the percentages of CD38 +, CD86 +, CD27 +, CD95 + and IL‐10 + B cells by flow cytometry. Serum IL‐10 concentration was examined by enzyme‐linked immunosorbent assay (ELISA). The percentages of CD38 + CD19 +, CD86 + CD19 +, CD38 + CD86 + CD19 +, and CD95 + CD19 + B cells were significantly higher in HBV‐MN patients than the HBV‐IT and HC. The percentages of CD5 + CD19 +, IL‐10 + CD19 + B cells and serum IL‐10 level in HBV‐MN patients were significantly higher than the HC, and lower than the HBV‐IT. Percentages of CD38 + CD19 +, and CD86 + CD19 + B cells were reduced after treatment, while the percentages of CD5 + CD1d + CD19 +, CD5 + CD1d + IL‐10 + CD19 +, and IL‐10 + CD19 + B cells were increased. The 24 h urinary protein concentration was positively correlated with the percentage of CD38 + CD19 +, and negatively correlated with the percentage of IL‐10 + CD19 + B cells and serum IL‐10 level. Similarly, the value of eGFR was negatively correlated with the percentage of CD38 + CD19 +, and positively correlated with the percentage of IL‐10 + CD19 + B cells and serum IL‐10 level. Serum IL‐10 level and the percentage of IL‐10 + CD19 + were negatively correlated with the percentages of CD38 + CD19 +, and CD86 + CD19 + B cells. These results suggest that CD86 + CD19 +, CD38 + CD86 + CD19 +, CD95 + CD19 +, and especially CD38 + CD19 + and IL‐10 + CD19 + cells may participate in the pathogenesis of HBV‐MN.