Efficient and Durable Vaccine against Intimin β of Diarrheagenic E. Coli Induced by Clay Nanoparticles. Issue 12 (27th January 2016) - Chen, Weiyu
Abstract : Improved strategies are urgently required to control infections with enterohemorrhagic Escherichia coli and enteropathogenic E. coli, two dominant zoonotic enteric pathogens responsible for a wide spectrum of illnesses as well as deaths of human being, with tremendous financial cost worldwide. The present study investigates the capacity of two clay nanoparticles (NPs) with opposite surface charges, namely synthetic layered double hydroxide (LDH) and hectorite (HEC) NPs as adjuvants to promote strong immune responses against the infections. Here both LDH and HEC NPs are showed to be able to carry an appreciable amount of Intimin β (1.1 and 4.4 mg per mg clay nanomaterials, respectively) and significantly facilitate antigen uptake by antigen‐presenting cells. Remarkably, these clay NPs induce strong antibody and cell‐mediated immune responses, which are much higher than that by the potent adjuvant, QuilA. Furthermore, these strong immune responses are well maintained for at least four months in the mouse model, during which there are no changes in histopathology of the animal organs. Collectively these data demonstrate the suitability of LDH and HEC NPs as useful adjuvants in new‐generation vaccine formulations to control various infectious diseases. Abstract : Two clay nanoparticles, namely layered double hydroxide and hectorite nanoparticles, are able to promote strong and sustained antibody and cell‐mediated immune responses against Intimin β (IB) for control of zoonotic enteric pathogen infections. Their adjuvant activity is much higher than that by the commonly used adjuvant QuilA, mainly attributed to the particular IB‐particle combination.