Tuning of glyconanomaterial shape and size for selective bacterial cell agglutination. Issue 11 (2nd March 2016)

• Record Type: Journal Article
• Authors: Cid Martín, J. J.; Assali, M.; Fernández-García, E.; Valdivia, V.; Sánchez-Fernández, E. M.; Garcia Fernández, J. M.; Wellinger, R. E.; Fernández, I.; Khiar, N.
• Is Part Of: Journal of materials chemistry. Volume 4:Issue 11(2016)
• Page Start: 2028
• Page End: 2037
• Publication Date: 2016-03-02
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c5tb02488a
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Acting as veritable glue, 1D-coated mannose carbon nanotubes efficiently and selectively regulate the agglutination and proliferation of the enterobacteria Escherichia coli type 1 fimbriae, much better than the mannose coated 3D-micelles. Abstract : Multivalent glycosystems are potential candidates for anti-adhesive therapy, a non-lethal approach against the ever increasing antibiotic resistance of pathogenic bacteria. In order to fine-tune the glyconanomaterial size and shape for selective bacterial cell agglutination, herein we report the synthesis of sugar-coated dynamic and polymeric 3D-micelles and 1D-carbon nanotubes. The reported shot-gun like synthetic approach is based on the ability of diacetylenic-based neoglycolipids to self-assemble into micelles in water and hierarchically self-assemble into hemimicelles on a single-walled carbon nanotube surface. The affinity of the nanosystems was preliminarily assessed by enzyme-linked lectin assay (ELLA) using the mannose-specific Concanavalin A lectin as a model receptor. Relative binding potency enhancements, compared to methyl α-d -mannopyranoside used as control, from 10- to 25- to 2340-folds in sugar molar basis were observed when passing from 3D dynamic micelles to static micelles, to 1D-mannose coated carbon nanotubes, respectively, indicative of a significant cluster glycoside effect. Importantly, these results were confirmed in vivo showing that the 1D-glyconanoring-coated carbon nanotubes efficiently and selectively regulate the agglutination and proliferation of the enterobacteria Escherichia coli type 1 fimbriae. These findings highlight the potential of sugar coated nano-materials as novel and effective tools in the control of bacterial pathogenesis.