Isotope effect analyses provide evidence for an altered transition state for RNA 2′-O-transphosphorylation catalyzed by Zn2+. Issue 24 (9th February 2016)

• Record Type: Journal Article
• Authors: Zhang, Shuming; Gu, Hong; Chen, Haoyuan; Strong, Emily; Ollie, Edward W.; Kellerman, Daniel; Liang, Danni; Miyagi, Masaru; Anderson, Vernon E.; Piccirilli, Joseph A.; York, Darrin M.; Harris, Michael E.
• Is Part Of: Chemical communications. Volume 52:Issue 24(2016)
• Page Start: 4462
• Page End: 4465
• Publication Date: 2016-02-09
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc10212j
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Solvent D2 O and 18 O kinetic isotope effects on RNA 2′- O -transphosphorylation catalyzed by Zn 2+ demonstrate an altered transition state relative to specific base catalysis. Abstract : Solvent D2 O and 18 O kinetic isotope effects on RNA 2′- O -transphosphorylation catalyzed by Zn 2+ demonstrate an altered transition state relative to specific base catalysis. A recent model from DFT calculations involving inner sphere coordination to the non-bridging and leaving group oxygens is consistent with the data.