Signal-on fluorescent sensor based on GQDs–MnO2 composite for glutathione. Issue 11 (26th February 2016)

• Record Type: Journal Article
• Authors: Liu, Zongen; Cai, Xiaohui; Lin, Xuefang; Zheng, Yanjie; Wu, Yangting; Chen, Pingping; Weng, Shaohuang; Lin, Liqing; Lin, Xinhua
• Is Part Of: Analytical methods. Volume 8:Issue 11(2016)
• Page Start: 2366
• Page End: 2374
• Publication Date: 2016-02-26
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c5ay03108g
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Facile, one-step strategy for selective GSH sensing in serum and in drug quality control using GQDs–MnO2 composite. Abstract : Graphene quantum dots (GQDs) exhibit strong photoluminescence, biocompatibility, and low toxicity and can easily functionalize surfaces to improve the detection of biomolecules. As-prepared MnO2 nanosheets were applied in the fabrication of GQDs–MnO2 composite to develop a signal-on fluorescent platform for sensing glutathione (GSH). The fluorescence of the GQDs was quenched by MnO2 in the GQDs–MnO2 composite as a result of fluorescence resonance energy transfer within the composite. The fluorescence of GQDs was restored when GSH was introduced because GSH reduced the MnO2 nanosheets into Mn 2+ and released GQDs. Under the optimized conditions, the concentration of GSH can be accurately detected with high sensitivity and specificity in Tris–HCl buffer and simulated serum. The proposed sensing strategy was successfully used to measure GSH in the range of 1–1000 μM with a detection limit of 0.45 μM. In addition, GSH in reduced glutathione injection and reduced glutathione tablets can be determined accurately using the proposed method. GQDs–MnO2 composite is thus a promising GSH fluorescence sensor for the analysis of complex systems and for quality control in the pharmaceutical industry.