Sodium tanshinone IIA sulfonate affects Marek's disease virus replication by inhibiting gB expression. (2nd April 2016)

• Record Type: Journal Article
• Authors: Wang, Jin; Sun, Panpan; Feng, Yuning; Guo, Jianhua; Sun, Yaogui; Lei, Haimin; Xu, Jianqin; Li, Hongquan
• Is Part Of: Pharmaceutical biology. Volume 54:Number 4(2016:Apr.)
• Page Start: 701
• Page End: 704
• Publication Date: 2016-04-02
• Subjects: Anti-MDV -- CEF cells -- traditional Chinese medicine; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2015.1072568
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context : Previous studies demonstrated that sodium tanshinone IIA sulfonate (STS) could inhibit MDV replication in vitro . The mechanism about how STS inhibits MDV replication is still not well understood. Objective : In this study, we evaluated the effect of STS on gB gene/protein of Marek's disease virus (MDV). Materials and methods : The concentration of 0.25 mg/ml of STS was used in this study. Meanwhile, 0.25 mg/ml of acyclovir (ACV) was used as a positive control. About 9–11-d-old embryonated specific-pathogen-free (SPF) chicken eggs were used to prepare CEF cells. CEF cells were infected with MDV 2 h, followed by treatment with STS. Real-time PCR and western blot assay were used to measure the gB (UL 27) gene/protein expression in STS treatment group at 24, 48, 72, and 96 h post-infection. Results : Compared with MDV control, the gB gene copies were significantly decreased in STS and ACV treatment groups at 72 h and 96 h ( p < 0.05), both in the DNA and in the mRNA level. Furthermore, the expression of gB protein was also inhibited by STS at 24, 72, and 96 h. Discussion and conclusion : Our study demonstrated that STS could effectively inhibit the MDV replication by suppressing gB gene/protein expression in cell culture.