A novel colorimetric/fluorescence dual-channel sensor based on NBD for the rapid and highly sensitive detection of cysteine and homocysteine in living cells. Issue 11 (29th February 2016)

• Record Type: Journal Article
• Authors: Shen, Youming; Zhang, Xiangyang; Zhang, Youyu; Zhang, Chunxiang; Jin, Junling; Li, Haitao; Yao, Shouzhuo
• Is Part Of: Analytical methods. Volume 8:Issue 11(2016)
• Page Start: 2420
• Page End: 2426
• Publication Date: 2016-02-29
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c5ay02884a
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A novel and simple turn-on fluorescent probe bearing NBD and benzaldehyde fragments for the rapid optical sensing of biothiols has been designed and synthesized. Abstract : A novel and simple turn-on fluorescent probe bearing 7-nitrobenz-2-oxa-1, 3-diazole and benzaldehyde fragments for the rapid optical sensing of cysteine and homocysteine has been designed and synthesized. The probe has almost no background fluorescence ( Φ f < 0.0001) in aqueous solutions; however, it displayed fluorescence turn-on response to cysteine and homocysteine and exhibited a large bathochromic-shift (106 nm) in absorption wavelength accompanied by color changes which could be distinguished by the naked-eye. There is a good linearity with cysteine in the range of 0 to 1.37 × 10 −4 M, with a detection limit of 98 nM (S/N = 3). In addition, the optical responses of the probe were investigated by density function theory (DFT) calculations. Moreover, it can be used to detect biological thiols in living cells.