Screening for and Identification of an Anti‐clam Vibrio Marine Bacterium from an Aquaculture Pond in the Yellow Sea. Issue 3 (29th December 2015)

• Record Type: Journal Article
• Authors: Sun, Xing; Zhao, Gengmao; Gu, Chenhao; Liu, Ling; Zhu, Ming; Liu, Zhaopu
• Is Part Of: Clean. Volume 44:Issue 3(2016:Mar.)
• Page Start: 304
• Page End: 308
• Publication Date: 2015-12-29
• Subjects: Fish disease -- Meretrix meretrix -- Probiotic -- 16S rDNA; Water quality -- Periodicals; Water -- Pollution -- Periodicals; Pollution -- Periodicals; Bioremediation -- Periodicals; Sewage -- Periodicals; Water chemistry -- Periodicals; 333.7205
• DOI: 10.1002/clen.201200478
• Languages: English
• ISSNs: 1863-0650

Abstract

Abstract: The identification and use of probiotic bacterial stains is a practical approach to protect clams grown in aquaculture farms from disease. The inhibition of the pathogenic bacterium Vibrio alginolyticus was used as a trait to select a candidate probiotic bacterial strain in this study. An ideal bacterial strain, SW‐1, was isolated from seawater from a clam farm. The selected isolate SW‐1 was identified based on its physiological, morphological, and biochemical characteristics and its 16S rDNA sequence. The experiments showed that strain SW‐1 had a high similarity to Pseudoalteromonas piscicida and could inhibit the growth of V. alginolyticus ( V .‐MP‐1). SW‐1 also improved the survival of clams following challenge with the pathogenic V .‐MP‐1. The mortality of clams was 100% after infection with 10 8 CFU/mL of V. alginolyticus, whereas mortality was only 11% when clams were infected with 10 8 CFU/mL of V .‐MP1 while simultaneously exposed to the same concentration of Pseudoalteromonas SW‐1, indicating that Pseudoalteromonas SW‐1 could be used as a probiotic to protect farmed clams, and thus reduce the effects of antibiotics on aquatic environment. Abstract : The results indicated that the Pseudoalteromonas SW‐1 strain has the properties of a biocontrol agent for use in clam culture farms. There is also a potential to use this strain for the control of Vibrio spp. in aquaculture systems and maintain the clam aquaculture sustainable development in the future.