An ultrafast turn-on thiol probe for protein labeling and bioimaging. Issue 6 (17th February 2016)
- Record Type:
- Journal Article
- Title:
- An ultrafast turn-on thiol probe for protein labeling and bioimaging. Issue 6 (17th February 2016)
- Main Title:
- An ultrafast turn-on thiol probe for protein labeling and bioimaging
- Authors:
- Sun, Jinyu
Zhang, Liangwei
Zhang, Xiaolong
Hu, Yuesong
Ge, Chunpo
Fang, Jianguo - Abstract:
- Abstract : A novel ultrafast turn-on thiol probe was developed that can be successfully applied to label protein thiols and imaging them in living cells. Abstract : A novel turn-on type of ultrafast biothiol fluorescent probe, Naph-EA-mal, was designed, synthesized and evaluated. The probe contains a naphthalimide moiety as a fluorophore, a maleimide unit as a thiol acceptor, and 1, 2-ethylenediamine as a linker.Naph-EA-mal displays high selectivity and a fast response toward thiols in aqueous solution. The reaction mechanism of the probe with thiols was confirmed by 1 H NMR and HRMS. Test strips were fabricated and a sharp color change was observed by the naked-eye. Furthermore, Naph-EA-mal was successfully applied to label protein thiols, image thiols in living cells, quantify thiol content in cells lysate, and determine the reversible protein thiols oxidation in fixed cells.
- Is Part Of:
- Analyst. Volume 141:Issue 6(2016)
- Journal:
- Analyst
- Issue:
- Volume 141:Issue 6(2016)
- Issue Display:
- Volume 141, Issue 6 (2016)
- Year:
- 2016
- Volume:
- 141
- Issue:
- 6
- Issue Sort Value:
- 2016-0141-0006-0000
- Page Start:
- 2009
- Page End:
- 2015
- Publication Date:
- 2016-02-17
- Subjects:
- Chemistry, Analytic -- Periodicals
543 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/an?e=1#!issueid=an139020&type=current&issnprint=0003-2654 ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/c5an02347e ↗
- Languages:
- English
- ISSNs:
- 0003-2654
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 0893.000000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 1136.xml