An ultrafast turn-on thiol probe for protein labeling and bioimaging. Issue 6 (17th February 2016)

• Record Type: Journal Article
• Authors: Sun, Jinyu; Zhang, Liangwei; Zhang, Xiaolong; Hu, Yuesong; Ge, Chunpo; Fang, Jianguo
• Is Part Of: Analyst. Volume 141:Issue 6(2016)
• Page Start: 2009
• Page End: 2015
• Publication Date: 2016-02-17
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c5an02347e
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A novel ultrafast turn-on thiol probe was developed that can be successfully applied to label protein thiols and imaging them in living cells. Abstract : A novel turn-on type of ultrafast biothiol fluorescent probe, Naph-EA-mal, was designed, synthesized and evaluated. The probe contains a naphthalimide moiety as a fluorophore, a maleimide unit as a thiol acceptor, and 1, 2-ethylenediamine as a linker.Naph-EA-mal displays high selectivity and a fast response toward thiols in aqueous solution. The reaction mechanism of the probe with thiols was confirmed by 1 H NMR and HRMS. Test strips were fabricated and a sharp color change was observed by the naked-eye. Furthermore, Naph-EA-mal was successfully applied to label protein thiols, image thiols in living cells, quantify thiol content in cells lysate, and determine the reversible protein thiols oxidation in fixed cells.