Effect of oxygen pressure during incubation with a 10B-carrier on 10B uptake capacity of cultured p53 wild-type and mutated tumor cells: dependency on p53 status of tumor cells and types of 10B-carriers. (2nd April 2016)

• Record Type: Journal Article
• Authors: Masunaga, Shin-ichiro; Tatebe, Hitoshi; Nishimura, Yasumasa; Tano, Keizo; Sanada, Yu; Moriwaki, Takahiro; Sakurai, Yoshinori; Tanaka, Hiroki; Suzuki, Minoru; Kondo, Natsuko; Maruhashi, Akira; Ono, Koji
• Is Part Of: International journal of radiation biology. Volume 92:Number 4(2016:Apr.)
• Page Start: 187
• Page End: 194
• Publication Date: 2016-04-02
• Subjects: Oxygen pressure -- boron neutron capture reaction -- 10B-carrier -- p53 status; Radiation -- Physiological effect -- Periodicals; Radiobiology -- Periodicals; 571.45
• DOI: 10.3109/09553002.2016.1137104
• Languages: English
• ISSNs: 0955-3002

Abstract

Abstract: Purpose To evaluate the effect of oxygen pressure during incubation with a 10 B-carrier on 10 B uptake capacity of cultured p53 wild -type and mutated tumor cells. Materials and methods Cultured human head and neck squamous cell carcinoma cell line transfected with mutant TP53 (SAS/ mp53 ), or with a neo vector as a control (SAS/ neo ) was incubated with L-para -boronophenylalanine- 10 B (BPA) or sodium mercaptoundecahydrododecaborate- 10 B (BSH) as a 10 B-carrier at the 10 B concentration of 60 ppm for 24 h under aerobic (20.7% of oxygen) or hypoxic (0.28% of oxygen) conditions. Immediately after incubation, cultured tumor cells received reactor thermal neutron beams, and a cell survival assay was performed. 10 B concentration of cultured SAS/ neo or SAS/ mp53 cells incubated under aerobic or hypoxic conditions was determined with a thermal neutron guide tube. Results Hypoxic incubation significantly decreased 10 B concentration of cultured cells with a clearer tendency observed following BPA than BSH treatment in both SAS/ neo and SAS/ mp53 cells. Following neutron beam irradiation, SAS/ mp53 cells showed significantly higher relative biological effectiveness values than SAS/ neo cells because of the significantly lower radiosensitivity of SAS/ mp53 to γ-rays than SAS/ neo cells. Conclusion Oxygen pressure during incubation with a 10 B-carrier had a critical impact on 10 B uptake of cultured tumor cells.