Nerve defect repair by differentiated adipose-derived stem cells and chondroitinase ABC-treated acellular nerves. (2nd June 2016)

• Record Type: Journal Article
• Authors: Jiang, Liangfu; Zheng, Yan; Chen, Ou; Chu, Tinggang; Ding, Jian; Yu, Qing
• Is Part Of: International journal of neuroscience. Volume 126:Number 6(2016:Jun.)
• Page Start: 568
• Page End: 576
• Publication Date: 2016-06-02
• Subjects: chondroitinase ABC -- adipose-derived stem cells -- acellular nerve -- nerve regeneration; Nervous system -- Periodicals; 612.805
• DOI: 10.3109/00207454.2015.1048547
• Languages: English
• ISSNs: 0020-7454

Abstract

Abstract : Objective : To evaluate the effects of differentiated adipose-derived stem cells (dADSC) and chondroitinase ABC (ChABC)-treated acellular nerves (ACN) in building artificial nerves and repairing nerve defects. Methods : ADSC were isolated from the adipose tissue of Wistar rats, induced to differentiate into Schwann-like cells, and implanted into ChABC-treated ACN to repair a 15-mm sciatic nerve defect in Sprague–Dawley rats (the experimental group, group D). The control groups were an autologous nerve transplantation group (group E); ACN (group A), ChABC-treated ACN graft group (group B), and dADSC + ACN (group C). Twelve weeks after surgery, electromyography recordings, tricep surae muscle wet weight recovery rate, and axon counts were measured to evaluate the repair of peripheral nerve defects. Results : The nerve conduction velocity, compound muscle action potentials, tricep surae muscle wet weight recovery rate, and myelinated axon counts in the ChABC-ACN/dADSC group were significantly higher than in the other groups ( P < 0.05), which were all lower than the autologous group ( P < 0.05). Conclusions : The combination of ChABC-treated ACN and dADSC exhibited a synergistic effect in promoting nerve regeneration, and could be an alternative for effective tissue-engineered nerves.