Pharyngeal arch artery defects and lethal malformations of the aortic arch and its branches in mice deficient for the Hrt1/Hey1 transcription factor. (February 2016)

• Record Type: Journal Article
• Authors: Fujita, Masahide; Sakabe, Masahide; Ioka, Tomoko; Watanabe, Yusuke; Kinugasa-Katayama, Yumi; Tsuchihashi, Takatoshi; Utset, Manuel F.; Yamagishi, Hiroyuki; Nakagawa, Osamu
• Is Part Of: Mechanisms of development. Volume 139(2016)
• Page Start: 65
• Page End: 73
• Publication Date: 2016-02
• Subjects: Cardiovascular development -- Hairy-related transcription factors -- Knockout mouse -- Pharyngeal arch artery defects -- Congenital vascular malformations -- Notch signaling; Developmental biology -- Periodicals; Molecular biology -- Periodicals; Developmental Biology -- Periodicals; Molecular Biology -- Periodicals; Biologie du développement -- Périodiques; Biologie moléculaire -- Périodiques; Developmental biology; Molecular biology; Periodicals; Electronic journals; 571.8
• DOI: 10.1016/j.mod.2015.11.002
• Languages: English
• ISSNs: 0925-4773

Abstract

Abstract: The aortic arch and major branch arteries are formed from the three pairs of pharyngeal arch arteries (PAAs) during embryonic development. Their morphological defects are clinically observed as isolated diseases, as a part of complicated cardiovascular anomalies or as a manifestation of multi-organ syndromes such as 22q11.2 deletion syndrome. Although numerous genes have been implicated in PAA formation and remodeling, detailed mechanisms remain poorly understood. Here we report that the mice null for Hrt1 / Hey1, a gene encoding a downstream transcription factor of Notch and ALK1 signaling pathways, show perinatal lethality on the C57BL/6N, C57BL/6N × C57BL/6J or C57BL/6N × 129X1/SvJ background. Hrt1 / Hey1 null embryos display abnormal development of the fourth PAA (PAA4), which results in congenital vascular defects including right-sided aortic arch, interruption of the aortic arch and aberrant origin of the right subclavian artery. Impaired vessel formation occurs randomly in PAA4 of Hrt1 / Hey1 null embryos, which likely causes the variability of congenital malformations. Endothelial cells in PAA4 of null embryos differentiate normally but are structurally disorganized at embryonic day 10.5 and 11.5. Vascular smooth muscle cells are nearly absent in the structurally-defective PAA4, despite the appropriate migration of cardiac neural crest cells into the fourth pharyngeal arches. Endothelial expression of Jag1 is down-regulated in the structurally-defective PAA4 of null embryos, which may be one of the mechanisms underlying the suppression of vascular smooth muscle cell differentiation. While the direct downstream phenomena of the Hrt1 / Hey1 deficiency remain to be clarified, we suggest that Hrt1/Hey1-dependent transcriptional regulation has an important role in PAA formation during embryonic development. Highlights: Hrt1/Hey1 null mice show perinatal lethality due to congenital malformations of the aortic arch and its branch arteries. The vascular defects in Hrt1/Hey1 null mice result from abnormal development of fourth pharyngeal arch artery (PAA4). Endothelial cells in PAA4 of Hrt1 / Hey1 null embryos differentiate normally but are structurally disorganized. Vascular smooth muscle cell differentiation is impaired in the structurally-defective PAA4 of Hrt1/Hey1 null embryos. Down-regulation of endothelial Jag1 expression may cause the loss of vascular smooth muscle cell differentiation.