Improvement of atopic dermatitis with topical application of Spirodela polyrhiza. (2nd March 2016)

• Record Type: Journal Article
• Authors: Lee, Hye Ji; Kim, Mi Hye; Choi, You Yeon; Kim, Eun Hye; Hong, Jongki; Kim, Kyuseok; Yang, Woong Mo
• Is Part Of: Journal of ethnopharmacology. Volume 180(2016)
• Page Start: 12
• Page End: 17
• Publication Date: 2016-03-02
• Subjects: Spirodela polyrhiza -- Atopic dermatitis -- IgE -- Cytokine; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2016.01.010
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Spirodela polyrhiza has been used as a traditional remedy for the treatment of urticarial, acute nephritis, inflammation, as well as skin disease. Aim of study: Atopic dermatitis (AD) is characterized hyperplasia of skin lesion and increase of serum immunoglobulin E (IgE) level. In this study, the topical effects of S. polyrhiza (SP) on 2, 4-dinitrochlorobenzene (DNCB)-induced AD mice model were investigated by several experiments. Materials and methods: BALB/c mice were randomly divided into five groups as NOR, CON, DEX, SP 1, and SP 100 groups ( n =5, respectively). To induce atopic dermatitis-like skin lesions, DNCB had been applied on shaved dorsal skin. SP was topically treated to DNCB-induced mice as 1 and 100 mg/mL concentrations. Histological changes were showed by hematoxylin and eosin (H&E) staining and the infiltration of mast cells was detected by toluidine blue staining. In addition, the level of IgE and each cytokines were measured and expressions of inflammatory signaling factors were analyzed by western blotting assay. Results: SP treatment improved a hyperplasia of epidermis and dermis in DNCB-induced AD-like skin lesion. The infiltration of mast cells was also decreased by treatment of SP. In addition, SP reduced the level of IgE in serum and attenuated the secretion of cytokines such as interleukin (IL)-4, IL-6, and tumor necrosis factor (TNF)-α. Treatment of SP also inhibited the expressions of pro-inflammatory mediators including nuclear factor-κB (NF-κB), phosphor-IκB-α, and mitogen-activated protein kinase (MAPK)s. Conclusions: From these data, we propose that SP ameliorates AD via modulation of pro-inflammatory mediators. SP may have the potential to be used as an alternative for treatment of AD. Graphical abstract: The leaves of Spirodela polyrhiza inhibited the expressions of pro-inflammatory mediators in atopic dermatitis-induced mice.