A CatalyCEST MRI Contrast Agent that Can Simultaneously Detect Two Enzyme Activities. (3rd February 2016)

• Record Type: Journal Article
• Authors: Fernández‐Cuervo, Gabriela; Sinharay, Sanhita; Pagel, Mark D.
• Is Part Of: Chembiochem. Volume 17:Number 5(2016)
• Page Start: 383
• Page End: 387
• Publication Date: 2016-02-03
• Subjects: enzyme catalysis -- exchange interactions -- hydrogen bonds -- imaging agents; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201500586
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: The simultaneous detection of multiple enzyme activities can improve the specificity of disease diagnoses. We therefore synthesized and characterized a diamagnetic chemical exchange saturation transfer (CEST) MRI contrast agent that can simultaneously detect two enzyme activities. Sulfatase and esterase enzymes cleave the ligands of the CEST agent, releasing salicylic acid that can be detected with CEST MRI. Importantly, both enzymes are required to activate the agent to produce a CEST MRI contrast, and the CEST agent was stable without enzyme treatment. These results established that this diamagnetic CEST MRI contrast agent is a platform technology with a modular design that can be potentially exploited to detect other combinations of enzyme activities, which can expand the armamentarium of contrast agents for molecular imaging. Abstract : In contrast : A diamagnetic chemical exchange saturation transfer (CEST) MRI contrast agent was converted to salicylic acid by sulfatase and esterase, which produces CEST MRI contrast after selective saturation at 9.25 ppm. This agent can be used with a catalyCEST MRI protocol to simultaneously detect two enzyme activities.