Screening for Oral Cancer Using Electrochemical Telomerase Assay. Issue 3 (26th August 2015)

• Record Type: Journal Article
• Authors: Hayakawa, Mana; Sato, Shinobu; Diala, Irmina; Kodama, Masaaki; Tomoeda‐Mori, Kumiko; Haraguchi, Kazuya; Tominaga, Kazuhiro; Takenaka, Shigeori
• Is Part Of: Electroanalysis. Volume 28:Issue 3(2016)
• Page Start: 503
• Page End: 507
• Publication Date: 2015-08-26
• Subjects: telomerase -- hTERT gene -- oral cancer screening -- electrochemical assay; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201500426
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: Electrochemical telomerase assay (ECTA) developed by our group was evaluated in an oral cancer screening using exfoliated oral cells and tissues obtained from patients of oral cancer, mucosa associated disease, or healthy volunteers. Telomerase activity from ECTA is correlated with hTERT mRNA expression level using a real‐time PCR and was increasing in the following order: healthy volunteer group<mucosa associated disease group<oral cancer group. Sensitivity and specificity of ECTA were 88 % and 72 %, respectively when used 17 % of the threshold value based on the receiver operating characteristic curve in ECTA data. Abstract :