Perturbation of nucleo‐cytoplasmic transport affects size of nucleus and nucleolus in human cells. Issue 5 (25th February 2016)

• Record Type: Journal Article
• Authors: Ganguly, Abira; Bhattacharjee, Chumki; Bhave, Madhura; Kailaje, Vaishali; Jain, Bhawik K.; Sengupta, Isha; Rangarajan, Annapoorni; Bhattacharyya, Dibyendu
• Is Part Of: FEBS letters. Volume 590:Issue 5(2016)
• Page Start: 631
• Page End: 643
• Publication Date: 2016-02-25
• Subjects: cancer -- importin beta -- karyoplasmic ratio -- nuclear transport -- nucleolus size -- nucleus size -- organelle size -- Ran; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.12077
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Size regulation of human cell nucleus and nucleolus are poorly understood subjects. 3D reconstruction of live image shows that the karyoplasmic ratio (KR) increases by 30–80% in transformed cell lines compared to their immortalized counterpart. The attenuation of nucleo‐cytoplasmic transport causes the KR value to increase by 30–50% in immortalized cell lines. Nucleolus volumes are significantly increased in transformed cell lines and the attenuation of nucleo‐cytoplasmic transport causes a significant increase in the nucleolus volume of immortalized cell lines. A cytosol and nuclear fraction swapping experiment emphasizes the potential role of unknown cytosolic factors in nuclear and nucleolar size regulation. Abstract :