Backbone assignment for minimal protein amounts of low structural homogeneity in the absence of deuteration. Issue 21 (25th January 2016)

• Record Type: Journal Article
• Authors: Xiang, ShengQi; Biernat, Jacek; Mandelkow, Eckhard; Becker, Stefan; Linser, Rasmus
• Is Part Of: Chemical communications. Volume 52:Issue 21(2016)
• Page Start: 4002
• Page End: 4005
• Publication Date: 2016-01-25
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc09160h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A set of higher-dimensionality 1 H-detected experiments is introduced for assigning non-deuterated proteins with low sample homogeneity at fast MAS. Abstract : NMR characterization of many proteins is limited by low expression, hurdles for deuteration, and poor sample homogeneity. We introduce a set of high-dimensionality proton-detected experiments developed for unambiguous resonance assignments of such proteins, which we could successfully apply to a 1 mg amount of non-deuterated Tau paired helical filaments.