Development of coupled supercritical fluid extraction-high performance liquid chromatography for quinone analysis in activated sludge. Issue 3 (11th February 2016)

• Record Type: Journal Article
• Authors: Juliasih, Ni Luh Gede Ratna; Yuan, Lee Chang; Atsuta, Yoichi; Daimon, Hiroyuki
• Is Part Of: Separation science and technology. Volume 51:Issue 3(2016)
• Page Start: 439
• Page End: 446
• Publication Date: 2016-02-11
• Subjects: Microbial community -- quinone profile -- SFE -- coupling technique -- activated sludge; Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2015.1086803
• Languages: English
• ISSNs: 0149-6395

Abstract

ABSTRACT: Coupled supercritical fluid extraction-high performance liquid chromatography (Coupled SFE-HPLC) was developed to determine the microbial community dynamics based on the quinone profile. It is a fast (less than 2 h) and high reproducibility (1.6% in coefficient of variation) method. The comparable results were obtained by the developed method and the organic solvent extraction method, it was 603.4 µmol kg −1 and 594.1 µmol kg −1 dry samples, respectively. The low dissimilarity index (0.07) between the two methods indicates that quinone profile obtained by both methods were considered similar. The results show that coupled SFE-HPLC method offers simplification for a rapid and routine analysis.