Induced thiacloprid insensitivity in honeybees (Apis mellifera L.) is associated with up‐regulation of detoxification genes. Issue 2 (20th January 2016)

• Record Type: Journal Article
• Authors: Alptekin, S.; Bass, C.; Nicholls, C.; Paine, M. J. I.; Clark, S. J.; Field, L.; Moores, G. D.
• Is Part Of: Insect molecular biology. Volume 25:Issue 2(2016:Apr.)
• Page Start: 171
• Page End: 180
• Publication Date: 2016-01-20
• Subjects: Apis mellifera -- induction -- P450 -- esterase -- thiacloprid -- metabolism; Insects -- Molecular aspects -- Periodicals; 595.7
• DOI: 10.1111/imb.12211
• Languages: English
• ISSNs: 0962-1075

Abstract

Abstract: Honey bees, Apis mellifera, are markedly less sensitive to neonicotinoid insecticides containing a cyanoimino pharmacophore than to those with a nitroimino group. Although previous work has suggested that this results from enhanced metabolism of the former by detoxification enzymes, the specific enzyme(s) involved remain to be characterized. In this work, a pretreatment of honey bees with a sublethal dose of thiacloprid resulted in induced insensitivity to the same compound immediately following thiacloprid feeding. A longer pretreatment time resulted in no, or increased, sensitivity. Transcriptome profiling, using microarrays, identified a number of genes encoding detoxification enzymes that were over‐expressed significantly in insecticide‐treated bees compared with untreated controls. These included five P450s, CYP6BE1, CYP305D1, CYP6AS5, CYP315A1, CYP301A1, and a carboxyl/cholinesterase (CCE) CCE8 . Four of these P450s were functionally expressed in Escherichia coli and their ability to metabolize thiacloprid examined by liquid chromatography‐mass spectrometry (LC‐MS) analysis.