Studying solutions at high shear rates: a dedicated microfluidics setup. (12th February 2016)

• Record Type: Journal Article
• Authors: Wieland, D. C. F.; Garamus, V. M.; Zander, T.; Krywka, C.; Wang, M.; Dedinaite, A.; Claesson, P. M.; Willumeit-Römer, R.
• Is Part Of: Journal of synchrotron radiation. Volume 23:Part 2(2016)
• Page Start: 480
• Page End: 486
• Publication Date: 2016-02-12
• Subjects: shear -- complex solutions -- small‐angle X‐ray scattering (SAXS) -- microfluidics -- lysozyme -- aggregation -- rheology -- protein interaction -- beamline setup; Synchrotron radiation -- Periodicals; Free electron lasers -- Periodicals; 539.73505
• DOI: 10.1107/S1600577515024856
• Languages: English
• ISSNs: 0909-0495

Abstract

Abstract : The development of a microfluidics setup for studying the interaction of complex solutions under varying shear conditions is presented. Abstract : The development of a dedicated small‐angle X‐ray scattering setup for the investigation of complex fluids at different controlled shear conditions is reported. The setup utilizes a microfluidics chip with a narrowing channel. As a consequence, a shear gradient is generated within the channel and the effect of shear rate on structure and interactions is mapped spatially. In a first experiment small‐angle X‐ray scattering is utilized to investigate highly concentrated protein solutions up to a shear rate of 300000 s −1 . These data demonstrate that equilibrium clusters of lysozyme are destabilized at high shear rates.