The inflammatory cytokine IL‐1β is involved in bladder remodeling after bladder outlet obstruction in mice. Issue 3 (3rd January 2015)

• Record Type: Journal Article
• Authors: Kanno, Yukiko; Mitsui, Takahiko; Kitta, Takeya; Moriya, Kimihiko; Tsukiyama, Tadasuke; Hatakeyama, Shigetsugu; Nonomura, Katsuya
• Is Part Of: Neurourology and urodynamics. Volume 35:Issue 3(2016:Mar.)
• Page Start: 377
• Page End: 381
• Publication Date: 2015-01-03
• Subjects: bladder outlet obstruction -- hypertrophy -- IGF‐1 -- IL‐1β -- inflammation -- remodeling; Urinary organs -- Periodicals; Urodynamics -- Periodicals; Urology -- Periodicals; 616.6
• DOI: 10.1002/nau.22721
• Languages: English
• ISSNs: 0733-2467

Abstract

Abstract: Aims: We investigated the relationship between IL‐1β and morphological and functional changes following partial bladder outlet obstruction (pBOO). Methods: Female wild‐type C57/BL6 mice (WT) and IL‐1β‐/‐ mice (KO) were used. Animals were sacrificed either 1 or 3 weeks after pBOO or sham surgery, and their bladders were harvested to determine bladder weight, for RT‐PCR to measure interleukin‐1β (IL‐1β), insulin growth factor‐1 (IGF‐1), and transforming growth factor‐β (TGF‐β) levels, and for histological analysis with Hematoxylin‐Eosin (HE) staining. Cystometry was performed on conscious animals 3 weeks after surgery to evaluate urodynamic parameters. IGF‐1 was also administered intraperitoneally to KO with pBOO, and bladder weight was then investigated. Results: IL‐1β‐mRNA levels were significantly higher in WT‐pBOO than in WT‐sham. IGF‐1‐mRNA and TGF‐β‐mRNA levels were also significantly higher in WT‐pBOO than in WT‐sham; however, these increases were smaller in KO‐pBOO than in WT‐pBOO. Bladder weight was significantly higher in WT‐pBOO than in WT‐sham, while increases in bladder weight were significantly suppressed in KO‐pBOO. HE staining revealed the thickened bladder wall in WT‐pBOO, and this phenomenon was less in KO‐pBOO than in WT‐pBOO. Regarding the urodynamic parameters examined, micturition pressure and bladder capacity were significantly higher in WT‐pBOO than in WT‐sham, but remained unchanged in KO‐pBOO. The administration of IGF‐1 to KO‐pBOO led to similar increases in bladder weight and the thickened bladder wall as those observed in WT‐pBOO. Conclusion: IL‐1β has the potential to induce bladder remodeling and deteriorate urodynamic parameters in pBOO. Neurourol. Urodynam. 35:377–381, 2016 . © 2015 Wiley Periodicals, Inc.