A sensitive and selective LC‐MS/MS method for the quantitative determination of segetalin A from the plasma of rats. (22nd September 2015)

• Record Type: Journal Article
• Authors: Wang, Jianmeng; Zhang, Nan; Pang, Li; Xu, Dahai; Yan, Weiwei; Wu, Jiang; Chen, Haiyan
• Is Part Of: Biomedical chromatography. Volume 30:Number 4(2016:Apr.)
• Page Start: 606
• Page End: 611
• Publication Date: 2015-09-22
• Subjects: Vaccaria segetalis -- cyclic hexapeptide -- segetalin A -- pharmacokinetic study; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3602
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: A sensitive and selective LC‐MS/MS method was developed and validated for the determination and pharmacokinetic investigation of segetalin A in rat plasma. Sample preparation was accomplished through a simple SPE procedure for the removal and preconcentration of the analyte and IS. Plasma samples were separated by HPLC on a Symmetry C18 column using a mobile phase consisting of methanol and 0.1% formic acid in water (70:30, v/v) with isocratic elution. The quantification was performed using multiple reaction monitoring with the transitions m/z 610.3 → 511.2 for segetalin A and m/z 779.4 → 751.4 for IS, respectively. The calibration curve was linear over the range of 8.0–4000 ng/mL with a limit of quantitation (LOQ) of 8.0 ng/mL. This method was applied in a pharmacokinetic study of segetalin A in rats. For intravenous (i.v.) administration, the plasma concentrations of segetalin A decreased quickly ( t 1/2 z, 1.31 ± 0.341 h). For oral administration, the plasma concentrations of segetalin A increased to a peak value at 1.50 ± 0.577 h, followed by a gradual decrease to the LOQ in 12 h. The mean AUC values after i.v. and oral administration were 553 ± 105 and 1482 ± 110 ng h/mL, respectively. Copyright © 2015 John Wiley & Sons, Ltd.