An enzyme–copper nanoparticle hybrid catalyst prepared from disassembly of an enzyme–inorganic nanocrystal three-dimensional nanostructure. Issue 25 (19th February 2016)

• Record Type: Journal Article
• Authors: Li, Zhixian; Ding, Yi; Wu, Xiaoling; Ge, Jun; Ouyang, Pingkai; Liu, Zheng
• Is Part Of: RSC advances. Volume 6:Issue 25(2016)
• Page Start: 20772
• Page End: 20776
• Publication Date: 2016-02-19
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra27904f
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Enzyme–copper nanoparticle hybrid catalysts were prepared with highly retained enzymatic and Cu-catalytic activities, enabling the chemo-enzymatic cascade reactions. Abstract : Enzyme–copper phosphate composites with highly porous three-dimentional (3D) structures were first prepared by self-assembly of protein molecules with copper ions in phosphate buffer solution. The self-assembled enzyme–copper phosphate 3D nanostructures were then disassembled by sodium borohydride reduction to produce enzyme-bound copper nanoparticles, which are regarded as 0D nanostructures. Based on the enzyme–copper phosphate composite, which has a high enzymatic activity, the direct transformation of copper phosphate into copper nanoparticles produced an enzyme–copper nanoparticle hybrid catalyst with both high enzymatic activity and copper-catalytic activity, serving as a novel type of hybrid catalyst for chemo-enzymatic cascade reactions.