A ratiometric fluorescence sensor for HOCl based on a FRET platform and application in living cells. Issue 21 (9th February 2016)

• Record Type: Journal Article
• Authors: Zhang, Yan-Ru; Zhao, Zhi-Min; Su, Le; Miao, Jun-Ying; Zhao, Bao-Xiang
• Is Part Of: RSC advances. Volume 6:Issue 21(2016)
• Page Start: 17059
• Page End: 17063
• Publication Date: 2016-02-09
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra26027b
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A ratiometric fluorescent probe CRSH based on a FRET platform for detecting HOCl. CRSH showed high selectivity, excellent sensitivity and a fast response toward HOCl. Abstract : Studies showed that the intravascular pH of neutrophils fell to 4.5–5.0 with stimulation for a few minutes. Under this condition, myeloperoxidase was activated to catalyze H2 O2 and Cl − to form hypochlorous acid (HOCl). Therefore, it is of significance to develop fluorescence probes for sensing HOCl in acid conditions. In this work, we reported a ratiometric probe CRSH based on a fluorescence resonance energy transfer (FRET) platform for detecting HOCl under acid conditions. Probe CRSH exhibited excellent sensitivity, high selectivity and a rapid response toward HOCl and is suitable for imaging endogenous HOCl in living cells.