Electrochemical sensing of guanine, adenine and 8-hydroxy-2′-deoxyguanosine at glassy carbon modified with single-walled carbon nanotubes covalently functionalized with lysine. Issue 16 (2nd February 2016)

• Record Type: Journal Article
• Authors: Gutiérrez, Alejandro; Gutierrez, Fabiana A.; Eguílaz, Marcos; González-Domínguez, José M.; Hernández-Ferrer, Javier; Ansón-Casaos, Alejandro; Martínez, María T.; Rivas, Gustavo A.
• Is Part Of: RSC advances. Volume 6:Issue 16(2016)
• Page Start: 13469
• Page End: 13477
• Publication Date: 2016-02-02
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra22556f
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Synthesis and characterization ofl -lysine covalently functionalized SWCNT and analytical application for the highly sensitive quantification of guanine, adenine and 8-hydroxy-2′-deoxyguanosine. Abstract : This work reports the synthesis and characterization of single-walled carbon nanotubes (SWCNT) covalently functionalized withl -lysine (Lys) and the analytical performance of glassy carbon electrodes (GCE) modified with a dispersion of SWCNT-Lys (GCE/SWCNT-Lys) for the highly sensitive quantification of guanine, adenine and 8-hydroxy-2′-deoxyguanosine. Detection limits of 75, 195 and 97 nM were obtained for guanine, adenine and 8-hydroxy-2′-deoxyguanosine, respectively by voltammetric adsorptive stripping with medium exchange. GCE/SWCNT-Lys was successfully used for the detection of adenine and guanine oxidation after adsorption of salmon sperm-double stranded DNA. A clear definition of 8-hydroxy-2′-deoxyguanosine oxidation signal is observed even in the presence of large excess of guanine, adenine or salmon sperm-double stranded DNA.