Dielectric passivation layer as a substratum on localized single-cell electroporation. Issue 13 (27th January 2016)

• Record Type: Journal Article
• Authors: Santra, Tuhin Subhra; Chen, Chih-Wei; Chang, Hwan-You; Tseng, Fan-Gang
• Is Part Of: RSC advances. Volume 6:Issue 13(2016)
• Page Start: 10979
• Page End: 10986
• Publication Date: 2016-01-27
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra18258a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Without dielectric passivation layer provide bulk electroporation, whereas with passivation layer generate an intense electric field to deliver molecules precisely into single cell, as name as localized single cell electroporation (LSCEP). Abstract : Single-cell electroporation is a powerful technique to understand cellular behavior with heterogeneity, which might be impossible based on bulk measurements of millions of cells together. In this study, a dielectric passivation layer was deposited on top of an indium-tin oxide micro-electrode-based transparent chip surface using a plasma enhanced chemical vapour deposition technique. We theoretically and experimentally investigated the key effects of the dielectric passivation layer on localized single-cell electroporation for different cancer cells, which were randomly distributed with a high density throughout the chip surface as a monolayer. The passivation layer not only prevented the conventional or bulk electroporation with bubble and ion generation, but also provide an intense electric field in-between electrode gap for localized single-cell electroporation with high cell viability. Thus, devices with dielectric passivation layers are potentially applicable for single-cell studies.