Ultrasensitive determination of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA by sheathless interfaced capillary electrophoresis–mass spectrometry. Issue 13 (12th January 2016)

• Record Type: Journal Article
• Authors: Yuan, Fang; Zhang, Xiao-Hui; Nie, Ji; Chen, Hong-Xu; Zhou, Ying-Lin; Zhang, Xin-Xiang
• Is Part Of: Chemical communications. Volume 52:Issue 13(2016)
• Page Start: 2698
• Page End: 2700
• Publication Date: 2016-01-12
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc10155g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : An ultrasensitive sheathless capillary electrophoresis–electrospray ionization–mass spectrometry system is developed for the determination of 5′-mdC and 5′-hmdC in only 125 pg (∼20 cells) genomic DNA samples. Abstract : A newly developed sheathless interface for capillary electrophoresis–mass spectrometry, using a porous tip sprayer, was first applied for highly sensitive determination of cytosine modifications. The system performed well in identification and quantification of both 5-methylcytosine and 5-hydroxymethylcytosine using only 125 pg (∼20 cells) genomic DNA samples.